TABLE 5.

Effects of external supply of pyruvate on the anaerobic fermentation profile of E. coli W3110GFA

Strain	Concn of initial carbon source (mM)		OD600	Concn of substrate or product (mM)a						Succinic acid ratioc	Foldd change
	Glucose	Pyruvate		Glucoseb	Succinic acid	Lactic acid	Formic acid	Acetic acid	Ethanol
W3110	50		1.79 ± 0.11	5.07 ± 0.45	2.43 ± 0.03	10.62 ± 2.42	88.03 ± 0.42	40.10 ± 0.20	5.77 ± 0.06	0.017	1.00
W3110GFA	50		0.73 ± 0.06	20.57 ± 3.02	8.16 ± 0.01	5.47 ± 0.49	27.47 ± 2.94	16.48 ± 1.55	1.88 ± 0.24	0.137	8.29
W3110GFA	25	50	1.40 ± 0.07	21.46 ± 2.07	3.28 ± 0.59	12.98 ± 0.51	41.02 ± 4.29	44.98 ± 1.31	5.50 ± 0.20	0.031	1.82
W3110GFA		100	1.62 ± 0.02	NDe	3.11 ± 0.05	14.66 ± 1.67	66.08 ± 3.51	80.43 ± 4.99	4.82 ± 1.24	0.018	1.06

^aAnaerobic vial fermentation for 24 h.

^bResidual glucose concentration.

^cCalculated as succinic acid/(succinic acid + lactic acid + formic acid + acetic acid + ethanol).

^dCalculated as succinic acid ratio/succinic acid ratio in the wild-type strain (0.017).

^eNot detected.