TABLE 1.

Oligonucleotide probes used in slot blot hybridizations

Probe		Target siteb	Sequence (5′ to 3′)	Wash temp (°C)	Target organisms	Reference
Namea	Abbreviation
S-*-Univ-1390-a-A-18	Univ1390	1390-1407	GACGGGCGGTGTGTACAA	44	All	43
S-G-Nsm-0156-a-A-19	Nsm156	156-174	TATTAGCACATCTTTCGAT	46	Nitrosomonas spp., Nitrosococcus mobilis	29
S-G-βAOB-1224-a-A-20	Nso1225	1224-1243	CGCCATTGTATTACGTGTGA	51	Betaproteobacterial ammonia-oxidizing bacteria	29
S-G-Nbac-1000-a-A-15	Nb1000	1000-1014	TGCGACCGGTCATGG	42	Nitrobacter spp.	29
S-G-Ntspa-0685-a-A-22	Ntspa685	664-685	CACCGGGAATTCCGCGCTCCTC	63	Nitrospira moscoviensis, Nitrospira marina	20

^aProbe names have been standardized according to the Oligonucleotide Probe Database (3).

^bE. coli numbering.