FIG. 2.

Bacterial growth (A and C) and 2,4-dinitrophenol uptake (B and D) in wild-type and mutant strains of R. capsulatus B10 growing under diazotrophic conditions (A and B) or with 5 mM ammonium chloride (C and D) in the presence of 150 μM 2,4-dinitrophenol. Bacterial growth and 2,4-dinitrophenol uptake were determined as indicated in Materials and Methods at the times indicated in the figure. •, wild-type strain; ▵, nprA mutant strain; □, nprB mutant strain; ○, nprA nprB mutant strain. Data correspond to a representative experiment which was repeated three times with variations of <10%.