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. 2005 Dec;71(12):8157–8164. doi: 10.1128/AEM.71.12.8157-8164.2005

TABLE 1.

Comparison of fluorescence intensities (mean value of triplicates) and hybridization efficiencies (mean value of triplicates) for one-step FISH and two-step FISH at different temperatures and hybridization timesa

FISH conditions Fluorescence intensity SD FISH efficiency (%) SD
Std 225.7 1.3 100.0 0.7
50°C, 30 min 205.6 0.8 82.1 1.6
55°C, 30 min 215.1 1.5 91.3 2.7
60°C, 20 min 213.4 0.7 64.7 0.1
60°C, 30 min 215.7 0.8 83.2 1.9
65°C, 20 min 196.2 0.2 86.1 4.1
65°C, 30 min 161.8 0.6 100.1 1.7
70°C, 15 min 199.5 3.5 83.9 4.0
70°C, 20 min 195.4 8.1 94.9 1.9
70°C, 30 min 192.0 11.7 98.3 6.5
75°C, 10 min 201.7 0.6 85.5 0.1
75°C, 20 min 209.9 0.2 91.3 2.7
75°C, 30 min 219.8 2.9 97.1 3.8
20°C, 20 minb 207.2 2.2 73.2 2.8
50°C, 15 minb 212.8 3.2 100.5 0.9
50°C, 20 minb 211.8 2.2 99.5 2.6
50°C, 25 minb 211.0 1.8 80.0 2.2
55°C, 20 minb 198.5 1.4 100.8 3.9
a

Data are illustrated in Fig. 3 and 5. The fluorescence intensities of hybridized E. coli cells were normalized to 0.75-μm beads, while the hybridization efficiencies were normalized to the cell counts for the standard conditions (Std) (46°C; 3 h).

b

Indicates pretreatment of sample at 90°C for 5 min.