TABLE 2.

Effects of temperature ramping on the sensitivity and broad specificity of the assay

Assay condition	Signal intensity (108 RLU)a
	With temp ramping	Without temp ramping
1 × 1012 molecules of rRNA	94.2 ± 6.07	31.6 ± 1.69
1 × 1010 molecules of rRNA	13.1 ± 2.16	2.67 ± 0.122
Probe-only control	1.45 ± 0.275	3.87 ± 0.527
Yeast rRNA negative control	1.5 ± 0.675	2.67 ± 0.33

^aValues are reported as means ± standard deviations for three replicates.