. 2005 Dec;71(12):8958–8962. doi: 10.1128/AEM.71.12.8958-8962.2005

TABLE 1.

pufM_rev primer compared to its corresponding region in aligned pufM sequences

PufM_rev primer (complement strand) for:	Codon encoding amino acid^a							No. of mismatches
	F	W	R	W	T	M	G
	TTC	TGG	CGC	TGG	ACC/G	ATG	GG
Cultured bacteria
Acidiphilium cryptum	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Bradyrhizobium ORS278	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Chloroflexus aurantiacus	`TTC`	`TGG`	`CGC`	`TGG`	`tgC`	`ATG`	`GG`	2
Ectothiorhodospira shaposhnikovii	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Jannaschia CCSI	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Lamprocystis purpurea	`TTg`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	1
Rhodobacter azotoformans	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Rhodobacter capsulatus	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Rhodobacter sphaeroides	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Rhodobacter veldkampii	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Rhodospirillum molischianum	`TTC`	`TGG`	`CGC`	`TGG`	`caG`	`cTt`	`GG`	4
Rhodospirillum rubrum	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Rhodovulum sulfidophilum	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Roseateles depolymerans	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Roseiflexus castenholzii	`TTC`	`TGG`	`CGC`	`TGG`	`gtG`	`ATG`	`GG`	2
Roseospirillum parvum 9301	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
Rhodopseudomonas palustris	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Blastochloris viridis	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATC`	`GG`	0
Rubrivivax gelatinosus	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Environmental sequences^b
DelRiver_fos13D03*	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
DelRiver_fos06H03*	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
EBAC000_29C02**	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
EBAC000_60D04**	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
EBAC000_65D09**	`TaC`	`TGG`	`CGC`	`TGG`	`tgC`	`ATG`	`GG`	3
eBACred25D05***	`TTC`	`TGG`	`CGC`	`TGG`	`ACA`	`ATG`	`GG`	1
IBEA_CTG_UAAQ029TR	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
IBEA_CTG_SZAFS75TR	`TTT`	`TGG`	`CGC`	`TGG`	`ACT`	`ATG`	`GG`	2
IBEA_CTG_SSBKC12TF	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
IBEA_CTG_SSAYW76TF	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
IBEA_CTG_SKBBG42TR	`TTT`	`TGG`	`AGA`	`TGG`	`ACA`	`ATG`	`GG`	4
IBEA_CTG_2156731	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
IBEA_CTG_2073229	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
IBEA_CTG_2058454	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
Sequences from this study
eBACmed94_waw	`TTT`	`TGG`	`CGT`	`TGG`	`ACA`	`ATG`	`GG`	3
eBACmed88_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
eBACmed26_waw	`TTT`	`TGG`	`CGT`	`TGG`	`ACA`	`ATG`	`GG`	3
eBACmed53C_waw	`TTC`	`TGG`	`CGC`	`TGG`	`ACG`	`ATG`	`GG`	0
eBACmed49G_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACT`	`ATG`	`GG`	3
eBACmed75G10_waw	`TTC`	`TGG`	`AGA`	`TGG`	`tgC`	`ATG`	`GG`	4
eBACmed19_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACG`	`ATG`	`GG`	2
eBACmed31B01	`TTT`	`TGG`	`CGT`	`TGG`	`ACA`	`ATG`	`GG`	3
envMED_0ma_waw	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
envMED_0mb_waw	`TTT`	`cGG`	`CGT`	`TGG`	`ACA`	`ATG`	`GG`	4
envMED_0mc_waw	`TTC`	`TGG`	`CGC`	`TGG`	`ACC`	`ATG`	`GG`	0
envMED_12m2_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
envMED_S06_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
envRED_7m4_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACC`	`ATG`	`GG`	2
envRED_30m_waw	`TTC`	`TGG`	`CGC`	`TGG`	`ACA`	`ATG`	`GG`	1
envRED_50m_waw	`TTC`	`TGG`	`AGA`	`TGG`	`ACT`	`ATG`	`GG`	3

Mismatches leading to missense mutations are shown in lowercase; mismatches leading to silent mutations are shown in bold.

*, fosmid clones retrieved by Waidner and Kirchman (18); **, clones retrieved by Béjà et al. (3); ***, clones retrieved by Oz et al. (12); IBEA_CTG, clones from Venter et al. (17).