TABLE 1.
Specific primer sequences designed for KB1 RDH genes
| KB1 gene target | Primer namea | Sequence (5′-3′) |
|---|---|---|
| rdhA1 | rdhA1_246f | ATCGGAGCTGCACAAGTAGG |
| rdhA1_336r | TCTTGTGAGCGGTGTCTTTG | |
| rdhA2 | rdhA2_720f | CAAAGGAGATGTTCCGGTGT |
| rdhA2_985f | CAGGTGGAAAAGACCGGTTA | |
| rdhA3 | rdhA3_1149f | CATTCTCCGGGAAGAAAACA |
| rdhA3_1379r | CCAGGCTTCCTTGTCTTCAG | |
| rdhA4 | rdhA4_754f | TTGTTATGCCGCCAATATGA |
| rdhA4_925r | TCTATCCATTTCGCCCAGAC | |
| rdhA5 | rdhA5_1017f | GATGCAGGCATTTACCGTTT |
| rdhA5_1137r | GTCTCTTTGCCTTCGGTCAG | |
| rdhA6 | rdhA6_318f | ATTTAGCGTGGGCAAAACAG |
| rdhA6_555r | CCTTCCCACCTTGGGTATTT | |
| rdhA7 | rdhA7_1391f | GCTAAAGAGCCGTCATCCTG |
| rdhA7_1539r | GCAGTAACAACAGCCCCAAT | |
| rdhA8 | rdhA8_845f | CCCAAGGTAGGTGTGCAGAT |
| rdhA8_1016r | CCCGGTTAGTTACCCCGTAT | |
| rdhA9 | rdhA9_251f | CTGACCTTGAAACCCCTGAA |
| rdhA9_425r | TTGCCACCCATTTCCATATT | |
| rdhA10 | rdhA10_710f | GCTGAAACACCCACCAAACT |
| rdhA10_860r | CGACAAAGGGGAATCTTTGA | |
| rdhA11 | rdhA11_429f | TAATGGCAACCGGAGGTAAG |
| rdhA11_609r | TCTACCGGTATGGCCTGAAC | |
| rdhA12 | rdhA12_864f | AGGAGTTCCTGTGGGGACTT |
| rdhA12_994r | TTTGGGGGTCATAACTGCTC | |
| rdhA13 | rdhA13_1356f | CAGGGTACCTGTCCCTTCAA |
| rdhA13_1493r | AGGGTTCTTCCGTCCGTACT | |
| rdhA14 | rdhA14_642f | GAAAGCTCAGCCGATGACTC |
| rdhA14_846r | TGGTTGAGGTAGGGTGAAGG |
a
The position numbers are shown within the names with respect to the first nucleotide of each gene following the RRF2 primer binding site. f, forward; r, reverse.