TABLE 3.
KB1 RDH gene | Most similar RDH gene | No. of clones containing each RDH gene transcript per treatmenta
|
|||
---|---|---|---|---|---|
TCE | cDCE | VC | 1,2-DCA | ||
rdhA6 | BAV1 bvcA | 5 | 3 | 4 | 3 |
rdhA14 | VS vcrA | 2 | 0 | 5 | 0 |
rdhA5 | FL2 rdhA6 | 3 | 7 | 1 | 12 |
rdhA3 | CBDB1 rdhA3 | 0 | 1 | 0 | 0 |
rdhA7 | CBDB1 rdhA14 | 0 | 1 | 0 | 0 |
rdhA1 | CBDB1 rdhA4 | 0 | 1 | 1 | 0 |
rdhA8 | FL2 rdhA8 | 0 | 1 | 0 | 0 |
Total | 10 | 14 | 11 | 15 |
For each chlorinated substrate, RNA was extracted at one time point soon after dechlorination began; RDH genes were amplified from cDNA samples, cloned, and sequenced. The number of occurrences of each RDH gene transcript from the different treatments is reported.