FIG. 4.

Unrooted phylogenetic tree of Wolbachia-like sequences based on 16S rRNA genes showing positions of members of the six Wolbachia supergroups A to F (41) and a representative clone from OTUs 1 and 2. The tree was generated using the neighbor-joining method with 10,000 bootstrap replicates using 1,290 nucleotides. Maximum-likelihood analysis resulted in the same tree topology (data not shown). Scientific names represent insect host species and are followed by GenBank accession numbers and the Wolbachia supergroup designations. The bar represents 0.01 substitutions per site. Using other clones to represent OTU 1 or OTU 2 did not affect tree topology.