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. 2005 Dec;71(12):8581–8586. doi: 10.1128/AEM.71.12.8581-8586.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — Confirmation of lipase display on the surface of P. putida KT2442. (A) Confirmation by immunoblotting of P. putida expressing OprF_t and OprF_t-lipase fusion protein. Lane 1, whole-cell lysate of P. putida; lane 2, whole-cell lysate of P. putida harboring pMO188PL; lane 3, outer membrane fraction of P. putida harboring pMO188PL. (B) Confirmation by flow cytometry analysis of P. putida and P. putida harboring pMO188PL. The arrow indicates the profile of recombinant P. putida(pMO188PL). The number of cells analyzed in each experiments was 50,000.