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. 2005 Dec;71(12):8581–8586. doi: 10.1128/AEM.71.12.8581-8586.2005

FIG. 3.

FIG. 3.

Stability of lipase displayed on the cell surface of E. coli XL-10 Gold harboring pTacO164PL and P. putida KT2442 harboring pMO188PL during prolonged incubation in organic solvents at 47°C. The enzyme activities were determined at 37°C and pH 8.0 using p-nitrophenyl decanoate as the substrate. The relative activity was calculated by assuming that the initial activity was 100%. ○, recombinant E. coli in hexane; (□, recombinant E. coli in isopropanol; •, recombinant P. putida in hexane; ▪, recombinant P. putida in isopropanol.