FIG. 1.

DNA maps of plasmids pART2 and pART3. The following plasmid features are presented: pCG100 and ColE1 origins of replication; Kan^r, kanamycin resistance gene; P, promoter/operator of the hdnO gene (hdnOp); MCS; His₈, eight-histidine tag coding sequence; and plasmid size in bp. Relevant restriction sites, including unique restriction sites, and bp positioning are represented. pART3 additionally carries the hdnO repressor gene (hnoR). The bottom sequence shows the following features: rbs, inferred ribosomal binding site; ATG, the translation start codon; MCS with nine unique restriction sites; coding sequence for a His₈ tag; and TAA, translation stop codon. Note that the SalI* site is present twice in the pART3 vector. DNA analysis and map displays were prepared with BioEdit (7).