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. 2005 Dec;71(12):8920–8924. doi: 10.1128/AEM.71.12.8920-8924.2005

FIG. 2.

FIG. 2.

Fluorescent A. nicotinovorans cells upon gfp expression. (A) Light microscopy image of A. nicotinovorans bacteria. Inset, magnified individual cell field. (B) Fluorescence microscopy image of A. nicotinovorans transformed with pART2-gfp; (C) A. nicotinovorans/pAO1 transformed with pART2-gfp; (D to F) A. nicotinovorans/pAO1 transformed with pART3-gfp and grown in the absence of nicotine (D), in the presence of nicotine for 60 min (E), and in the presence of nicotine overnight (F). Bacteria shown in panels A to C were grown in LB medium, and bacteria shown in panels D to F were grown in citrate medium supplemented with 0.5% yeast extract, mineral salts (5), and, as required, 0.05% l-nicotine. Pictures of A. nicotinovorans bacteria were taken using an Zeiss Axioskop 50 epifluorescence microscope equipped with a Plan-Nofluar 100× (1.3-numerical-aperture) objective and a triple-pass filter set. Digital images were recorded with a Nikon D100 camera.