TABLE 5.

Effect of incubation period on CGP synthesis in E. coli DH1(pMa/c5-914::cphA₆₈₀₃)^a

Incubation period (h)	Cell density (g CDM/liter)	CGP content of cells (% of CDM)
24	2.40 ± 0.35	28.9 ± 3.70
46	3.67 ± 0.46	27.7 ± 0.09
71	3.50 ± 0.44	27.6 ± 1.45

^aCells of E. coli DH1(pMa/c5-914::cphA₆₈₀₃) were grown in 250-ml Erlenmeyer flasks without baffles which contained 100 ml 6% (vol/vol) protamylasse supplemented with 100 μg/ml ampicillin. The initial pH was adjusted to 7.5 before sterilization, and the flasks were incubated at 37°C. Each day, one sample was taken and analyzed for CDM and CGP content as described in Materials and Methods. Data shown represent mean values and standard deviations of three independent experiments.