FIG. 2.

Phylogenetic positions of the endosymbiotic bacteria of the Pseudotrichonympha protists. The tree was inferred by the ML method based on comparisons of 1,254 unambiguously aligned positions of 16S rRNA gene sequences. The numbers at the nodes indicate the levels of support for ML, Bayesian inference, and maximum-parsimony results (from left to right, divided by slashes). Scale bar = 0.1 nucleotide substitution per position. Cluster V of the Bacteroidales sequences from termite guts is indicated by the vertical bar on the right. Sequences from termite guts are indicated by the prefixes Rs (Reticulitermes speratus), BCf (Coptotermes formosanus), and COB (Cubitermes orthognathus). The sequences obtained in this work and the sequences reported to be sequences of ectosymbionts of protists in other termites are indicated by boldface type. The database accession numbers are indicated after the names of taxa. Abbreviations: B., Bacteroides; Po., Porphyromonas; Pr., Prevotella; D., Dysgonomonas.