TABLE 1.
PCR primers used in this study
| Primer | Target gene | Sequence (5′ to 3′) | Reference |
|---|---|---|---|
| Eub27F | 16S rRNA | ATTGGATCCGTTTGATCMTGGCTCAG | 18 |
| 1392R | 16S rRNA | CGGGCGGTGTGTRC | 18 |
| gyrB-UP1A | gyrB | CAYGCNGGNGGNAARTTYGA | 36a |
| gyrB-UP2A | gyrB | CCRTCNACRTCNGCRTCNGTCAT | 36a |
| EB-EFTu-Fw1 | EF-Tu | GCXGAYTAYRTXAARAAYATG | This study |
| EB-EFTu-Rv1 | EF-Tu | TGXCKXCCXCCYTCXTCYTT | This study |
| groEL-F | groEL | GCNCCIGAYGGNACNACNAC | This study |
| groEL-R | groEL | CCRAAICCNGGNGCYTTNAC | This study |
a
Primers gyrB-UP1A and gyrB-UP2A were slightly modified from the primers described in a previous report (36).