TABLE 2.
Summary of E. coli O86:B7 O-antigen biosynthesis genes
| ORFa | Gene | No. of amino acids | % G+C content | Similar protein (Genbank accession no.) | % Identical/ % similar (no. of amino acid overlap) | Putative function |
|---|---|---|---|---|---|---|
| 1 | gne | 339 | 33.2 | Gne of Yersinia enterocolitica O:8 (AAC60777) | 57/72 (337) | UDP-N-acetylglucosamine-4-epimerase |
| 2 | wbwH | 338 | 34.2 | WbcQ of Yersinia enterocolitica (CAA87705) | 47/68 (337) | Glycosyltransferase |
| 3 | gmd | 373 | 52.6 | GDP-mannose 4,6-dehydratase of E. coli O128 (AA037691) | 95/98 (370) | GDP-D-mannose dehydratase |
| 4 | fcl | 321 | 56.1 | GDP-fucose synthetase of E. coli O128 (AA037692) | 99/99 (321) | GDP-fucose synthetase |
| 5 | gmm | 167 | 52.4 | GDP-mannose mannosyl hydrolase of E. coli O128 (AA037693) | 94/97 (167) | GDP-mannose mannosyl hydrolase |
| 6 | manC | 482 | 36.4 | GDP-mannose pyrophosphorylase of E. coli O128 (AA037694) | 85/93 (482) | GDP-mannose pyrophosphorylase |
| 7 | manB | 462 | 50.5 | Phosphomannomutase of E. coli O128 (AA037695) | 92/95 (455) | Phosphomannomutase |
| 8 | wzx | 400 | 27.8 | O-antigen flippase of Salmonella enterica (AAV34509) | 16/38 (218) | O-antigen flippase |
| 9 | wbwI | 234 | 28.4 | Glycosyltransferase of Homo sapiens (AAQ88542) | 23/42 (191) | Glycosyltransferase |
| 10 | wzy | 466 | 28.8 | O-antigen polymerase of E. coli O111 (AAD46730) | 25/44 (191) | O-antigen polymerase |
| 11 | wbwJ | 247 | 31.2 | WbgO of E. coli O55 (AAL67559) | 39/59 (217) | Glycosyltransferase |
| 12 | wbwK | 302 | 32.6 | Fucosyltransferase of E. coli O128 (AA037698) | 32/53 (300) | Glycosyltransferase |
| 13 | gnd | 432 | 51.7 | Gnd of E. coli K-12 (AAC75090) | 99/99 (374) | Gluconate 6-P dehydrogenase |
| 14 | ugd | 388 | 43.4 | UDP-glucose 6-dehydrogenase of E. coli O128 (AA037704) | 98/99 (388) | UDP-glucose dehydrogenase |
| 15 | wzz | 337 | 46.1 | Rol of Shigella flexneri (CAA50783) | 98/99 (317) | O-antigen chain length determinant |
a
ORF, open reading frame.