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(A) Hemin binding by whole cells from different strains of V. anguillarum cultured under iron-rich (1) and iron-deficient (2) conditions. (B) Effect of proteinase K treatment (2) on hemin binding compared to nontreated cells (1). Lanes: A, E. coli HB101 (negative control); B, 775; C, RV22; D, ET-208; E, RM 40.1; F, ATCC 43309; G, ATCC 43310; H, ATCC 43311; I, ATCC 43313.
