TABLE 2.
Bacterial strains of V. anguillarum used in this study, utilization of hemin as the only iron source, and presence of huvA, huvBCD, and huvS genes
| Strain | Serotype | Source, location | Utilization of Hma | Presence ofb:
|
||
|---|---|---|---|---|---|---|
| huvA | huvBCD | huvS | ||||
| R82 | O1 | Scophthalmus maximus, Spain | + | + | + | − |
| ATCCc 43305 | O1 | Oncorhynchus mykiss, Denmark | + | + | + | − |
| 775 | O1 | Oncorhynchus kisutch, United States | + | + | + | − |
| TM-14 | O1 | Oncorhynchus mykiss, Spain | + | + | + | − |
| 96F | O1 | Morone saxatilis, United States | + | + | + | − |
| RV22 | O2 | Scophthalmus maximus, Spain | + | + | + | − |
| ATCC 43306 | O2 | Gadus morhua, Denmark | + | − | + | + |
| ATCC 14181 | O2 | Gadus morhua, Denmark | + | + | + | − |
| 43F | O2 | Morone saxatilis, United States | + | + | + | − |
| 13A5 | O3 | Seawater, Spain | + | − | − | − |
| B.1.1.2/4 | O3 | Seawater, Denmark | + | − | − | − |
| ET-208 | O3 | Anguilla japonica, Japan | + | − | + | + |
| 11008 | O3 | Dicentrarchus labrax, France | + | − | + | + |
| ATCC 43307 | O3 | Oncorhynchus mykiss, Denmark | + | − | + | + |
| PT-493 | O3 | Plecoglossus altivelis, Japan | + | − | + | + |
| RPM 41.11 | O4 | Scophthalmus maximus, Spain | + | + | + | − |
| ATCC 43308 | O4 | Gadus morhua, Denmark | + | − | + | + |
| ATCC 43309 | O5 | Gadus morhua, Denmark | + | + | + | − |
| ATCC 43310 | O6 | Gadus morhua, Denmark | + | − | + | + |
| ATCC 43311 | O7 | Anguilla anguilla, Denmark | + | − | + | + |
| ATCC 43312 | O8 | Gadus morhua, Denmark | + | + | + | − |
| ATCC 43313 | O9 | Gadus morhua, Denmark | + | + | + | − |
| ATCC 43314 | O10 | Gadus morhua, Denmark | + | − | + | + |
a
Utilization of hemin (Hm) at 10 μM as the only iron source tested in CM9 plates containing EDDA. All strains were also positive in the utilization of Hemoglobin as iron source.
b
Presence detected by PCR and confirmed by Southern blot hybridization.
c
ATCC, American Type Culture Collection, Manassas, Va.