Figure 3. Differential Methylation Analysis Reveals Enrichment of Genes Related to Immune Response, Cell Cycle, Metabolic Genes in the Active RE Subset.

Volcano plot showing differentially methylated RE-associated CpGs between the Active and Repressed subtypes (|log₂FC| > 1 and FDR < 0.05). Twenty-five positions were significantly altered, including 22 hypomethylated and 3 hypermethylated loci. Highlighted DMPs (pink) map to genes involved in immune activation (PIK3CD, CD81, HLA-E), cell-cycle regulation (RASSF1), EMT (ELMO1, RAB11FIP3), and metabolic pathways (SUCLG2-DT, HCG17, RPS18).