FIG. 5.

Expression of full-length tPA by the Tat pathway. Equivalent numbers of FÅ113/pTrcTorAtPA cells grown in shake flasks and fermentors were harvested 6 and 10 h, respectively, after induction and fractionated into cytoplasmic (c) (open bars) and periplasmic (p) (gray bars) fractions. The plasminogen activation data shown are the averages of three replicate experiments. Relative activity is expressed as the activity in the cytoplasmic or periplasmic samples normalized to the activity measured in the cytoplasm or periplasm of FÅ113 cells expressing Δss-tPA in shake flasks or fermentors. Only data from fractionation experiments in which ≥95% of the β-galactosidase activity was in the cytoplasmic fraction are shown.