TABLE 2.
Summary of vtPA expression in various strains of E. colia
| Strain/plasmid | Relative cytoplasmic vtPA activityb | Relative periplasmic vtPA activityc |
|---|---|---|
| FÅ113/pTrcvtPA-h6 | 1.0 | 1.0 |
| FÅ113/pTrcStIIvtPA-h6 | 3.0 | 2.2 |
| FÅ113/pTrcTorAvtPA-h6 | 10.1 | 5.5 |
| FÅ113/pTrcTorAvtPA-h6/pABS-tatABC | 6.5 | 2.8 |
| FUDDY/pTrcTorAvtPA-h6 | 5.6 | 0.8 |
| DR473/pTrcTorAvtPA-h6 | 8.9 | 4.6 |
| DRA/pTrcTorAvtPA-h6 | 8.7 | 3.9 |
a
All vtPA activity values are the averages of three replicate experiments (standard error, <0.5%).
b
The relative cytoplasmic activity for all samples was normalized to the activity measured in the cytoplasm of FÅ113 cells expressing Δss-vtPA from pTrcvtPA-h6.
c
The relative periplasmic activity for all samples was normalized to the activity measured in the periplasm of FÅ113 cells expressing Δss-vtPA from pTrcvtPA-h6.