FIG. 1.

Nucleotide sequence of ORF1 (boldface type) and the upstream region, showing the locations of the copper box homology (bases enclosed in boxes) and the divergent translational start of ORF3 [(←CAT)]. Oligonucleotide primers used for subcloning and primer extension are underlined. Bases added to primers for generation of new restriction sites are indicated by lowercase superscript letters. The position of the Tn3 insertion in ORF1 marker exchange mutant ME5 is indicated by an inverted solid triangle.