FIG. 2.

(A) Restriction map of pXv26Cu-2, a 7,652-bp region of DNA that confers resistance to copper in XvP26. The open arrows, arrows with bars, and solid arrows indicate transposon insertions that result in transconjugants in 91-118 (copper-sensitive strain) that are resistant to copper, have intermediate sensitivity to copper, and are sensitive to copper, respectively. (B) Subclone of pXv26Cu-2 in pBluescript used to construct fusion clones shown in panels C and D. (C and D) Reporter constructs for measuring GUS activity for copper induction of ORF3 and ORF4, respectively. Construction of additional reporter clones pΔKXORF3uidA and pORF1uidA (not shown) is described in detail in Materials and Methods.