TABLE 2.
Effect of copper on induction of ORF3 and ORF4 in 91-118, XvP26, and XvP26 marker exchange transconjugant backgrounds
| Recipient | β-Glucuronidase activity (U)a
|
|||||
|---|---|---|---|---|---|---|
| pORF3uidA
|
pΔKXORF3uidA
|
pORF4uidA
|
||||
| Without Cu | With Cu | Without Cu | With Cu | Without Cu | With Cu | |
| 91-118 | 41 ± 2 | 245 ± 11 | 0 | 0 | 160 ± 6 | 416 ± 10 |
| XvP26 | 14 ± 1 | 31 ± 1 | 216 ± 6 | 220 ± 7 | 31 ± 2 | 73 ± 4 |
| ME5 | 44 ± 2 | 133 ± 2 | 0 | 0 | 105 ± 1 | 268 ± 1 |
| ME12 | 116 ± 2 | 233 ± 5 | 72 ± 1 | 73 ± 2 | 116 ± 5 | 249 ± 9 |
| ME25 | 100 ± 3 | 226 ± 8 | 278 ± 10 | 319 ± 2 | 105 ± 1 | 331 ± 2 |
| ME23 | 70 ± 2 | 172 ± 7 | 0 | 43 ± 2 | 36 ± 1 | 197 ± 4 |
| ME16 | 59 ± 1 | 243 ± 8 | 0 | 56 ± 1 | 59 ± 3 | 331 ± 1 |
a
β-Glucuronidase activity is expressed in units (nanomoles of MU produced per minute)/1010 CFU after 3 h of induction in CYE broth with or without copper.