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. 2005 Dec;71(12):7866–7871. doi: 10.1128/AEM.71.12.7866-7871.2005

TABLE 2.

Measured average in vivo rates of specific conversion of furfural and HMF and in vitro enzyme activitiesa

Compound Strain Mode In vivo conversion rate (g g biomass−1 h−1) NADH-dependent in vitro enzyme activity (g g biomass−1 h−1)b NADPH-dependent in vitro enzyme activity (g g biomass−1 h−1)b Total in vitro enzyme activity (g g biomass−1 h−1)b
Furfural CBS 8066 Fed-batch 0.009 0.63 0.041 0.67
TMB3000 Fed-batch 0.012 1.8 0.21 2.0
HMF CBS 8066 Fed-batch 0.034 0.004 0.029 0.033
TMB3000 Fed-batch 0.08 0.78 0.14 0.92
a

The conversion rates were calculated based on consumption of furfural or HMF during the feeding of hydrolysate. The in vitro furfural- and HMF-reducing activities were measured as described previously (23).

b

The in vitro activities were recalculated as g furfural or HMF converted g biomass−1 h−1. This was done to enable a comparison between the conversion rates in the fermentations and the in vitro measurements.