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Assay setup. The sample is preincubated with monoclonal anti-BoNT/C and D and biotinylated polyclonal anti-BoNT/C and D (anti-BoNT/C+D) antibodies. During preincubation, a complex with BoNT is formed, which is captured by the anti-mouse IgG coated on the assay disk of the column. Streptavidin-poly-HRP binds to the biotinylated antibody and precipitates the substrate on the assay disk.
