Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Variations of antibody concentrations and incubation times and their effect on the sensitivity and assay performance for the detection of BoNT/C and D. The best assay sensitivity is achieved with 0.3 μg ml−1 monoclonal antibodies and 0.1 μg ml−1 biotinylated polyclonal antibodies for BoNT/C and D. With BoNT/D, 4-h incubations showed the highest sensitivity; with BoNT/C, 16-h incubations showed the highest sensitivity.
