FIG. 1.

Deletion of A. fumigatus rasB. (A) Graphical representation of the rasB genomic locus from the wild-type, ΔrasB, and reconstituted strains (ΔrasB+rasB). Probes (P) used for Southern blotting are shown as small black boxes. Relative positions of the BamHI restriction sites are given. (B) Genomic Southern blot of wild-type, ΔrasB, and ΔrasB+rasB DNA digested with BamHI. (C) Reverse transcription-PCR of the isogenic set, showing loss and recovery of rasB message RNA in the ΔrasB and reconstituted strains, respectively. The loading control is A. fumigatus gpdA.