FIG. 3.

Digital confocal images of mock-infected or HSV-1 (F)-infected HEp-2 cells immunostained with PKC-specific antibodies. At 16 h postinfection, cells were fixed with methanol and incubated with anti-lamin B antibody, anti-ICP4 antibody, and one of the following PKC-specific antibodies: anti-PKCα (A to D), anti-PKCδ (E to H), anti-PKCζ (I to L). Anti-PKC antibodies were stained with FITC-conjugated secondary antibody (green channel), anti-lamin B antibody was stained with Texas Red-conjugated secondary antibody (red channel), and ICP4 was stained with Cy5-conjugated secondary antibody (blue channel).