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Ectopic rescue of M53 deletion mutant. The growth kinetics of the viruses derived from pSMfr3 (wt) or pM53E (M53E) were determined on NIH 3T3 cells. Subconfluent cultures were infected with the respective viruses. Supernatants of the infected cells were harvested on the indicated days, and the infectious progeny was quantified by a plaque assay.
