Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescence emission spectra of thioflavin T in the presence of NSP4 mutant proteins. Excitation wavelength was 450 nm. Total protein used for each of the mutants was 25 μmol. Only in the presence of ΔN72, ThT exhibited a large increase (>60-fold) in fluorescence compared to other mutants. Note that the single-amino acid mutant, Hgm3 (F76S), showed about 50% and the triple-amino acid mutant of DIR (dirm3) about 10% of fluorescence of that of ΔN72.
