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. 2006 Jan;80(1):412–425. doi: 10.1128/JVI.80.1.412-425.2006

FIG. 8.

FIG. 8.

Differential susceptibility of Hg18 and SA11 NSP4 mutant proteins to trypsin cleavage. (A) Tricine-SDS-PAGE of the trypsin cleavage products. Note the 9.95-kDa trypsin-resistant fragment derived from ΔN72. Note the significant resistance of the DIR mutants to trypsin cleavage. The conditions and time period of incubation are indicate above the gels. Similar results were obtained using corresponding mutants of SA11 (data not shown). (B) Mass spectra of trypsin cleavage products of NSP4 mutants. Time-dependent conversion of the 10.56-kDa primary cleavage product to 9.95-kDa stable product between 5 min and 1 h is shown for ΔN72. The corresponding products of ΔN85 and ΔN94 are seen only at very early time points of trypsin treatment.