FIG. 9.
Protection from trypsin cleavage of the region N terminus to aa 146 in ΔN72. (A) Diagramatic representation of the predicted trypsin cleavage sites (arrows) on NSP4ΔN72 of SA11 (7, 12). His tag (H6) at the N terminus is indicated. Previously mapped C-terminal boundary of trypsin cleavage at aa 146 on SA11 NSP4 is indicated by *. For the amino acid sequence of this trypsin-resistant region, refer to Fig. 4A. (B) Western blotting and detection of the trypsin-resistant fragments of Hg18ΔN72 and Hg18ΔN85 using mouse anti-His horseradish peroxidase-conjugated antibody (QIAGEN).