TABLE 1.
Description of various deletion and amino acid substitution mutants of NSP4
| Mutant | Mutation at:
|
||
|---|---|---|---|
| N terminus | DIR | C terminus | |
| N-terminal deletion | |||
| ΔN47 | Lacks 47 aa | Wild type | Wild type |
| ΔN57 | Lacks 57 aa | Wild type | Wild type |
| ΔN72 | Lacks 72 aa | Wild type | Wild type |
| ΔN85 | Lacks 85 aa | Wild type | Wild type |
| ΔN94 | Lacks 94 aa | Wild type | Wild type |
| Deletion in ΔN72 AAH73-85 | |||
| ΔN77 | Lacks aa 73-77 | Wild type | Wild type |
| ΔN80 | Lacks aa 73-80 | Wild type | Wild type |
| ΔN83 | Lacks aa 73-83 | Wild type | Wild type |
| C-terminal mutants of ΔN72 | |||
| Hgm6 | As in ΔN72 | Wild type | 5-aa deletion |
| Hgm15 (M175I) | As in ΔN72 | Wild type | Met to Val |
| Amino acid substitutions in ΔN72 AAH73-85 | |||
| Hgm 1 | 6-aa substitution (I75A-F76S-N77D-T78A-L79S-L80A) | Wild type | Wild type |
| Hgm 3 | (F76S) | Wild type | Wild type |
| Mutations in ΔN72 DIR | |||
| SA11-dirm1 | As in ΔN72 | (T139S-G140A) | Wild type |
| SA11-dirm2 | As in ΔN72 | (Y131S-T139S) | Wild type |
| SA11-dirm3 | As in ΔN72 | (Y131S-T139S-G140A) | Wild type |
| SA11-dirm4 | As in ΔN72 | (T139S) | Wild type |