FIG. 10.

Release of virus-like particles from cells expressing HIV Gag constructs. (A) Schematic representation of the HIV Gag construct H6Tko, which carries a knockout mutation in the PTAP motif, and the H6Ako construct, which lacks the binding site for Alix. The late-domain motifs of BTV NS3 and Ebola virus VP40 were appended to the C termini of both constructs, as indicated in the lower panel. Numbers indicate amino acid positions. (B) Gag fusion constructs and parent plasmids were transfected into 293T cells, and VLP release was monitored as described in the legend to Fig. 8. (C) Whole-cell lysates of transfected cells were analyzed with Gag antiserum to verify that the constructs were expressed at equal levels.