FIG. 4.

Nuclear egress of capsids into the cytoplasm. The percentage of living cells displaying cytoplasmic capsids 11 to 15 h postinfection is shown. Vero cells were infected with PRV-GS575, having intact UL36 and UL37 genes (“WT”), PRV-GS678 (ΔUL36), PRV-GS993 (ΔUL37), the PRV-GS678R revertant virus (UL36R), or the PRV-GS993R revertant virus (UL37R) at an MOI of ≤0.1. Because capsids near the nuclear rim could not be scored easily in this assay, cells were only counted as positive if at least 10 fluorescent-capsid punctae were observed in the cytoplasm. Error bars are standard error of the proportions (SEp). PRV-GS678 and PRV-GS993 were significantly different from each other and from each revertant virus (z < 0.01).