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B2 is required for maximal accumulation of RNA1 in a variety of cell types. RNA1 (•) or RNA1ΔB2 (▪) transcripts were transfected into SB (A), BSR T7/5 (B), DF-1 (C), or HeLa (D) cells, and RNA1 accumulation was monitored over time by real-time RT-PCR, normalized against cellular 18S rRNA levels. Values shown are the means of three independent determinations, performed in duplicate. Error bars indicate the standard error of the mean.
