FIG. 2.

Codetection of RSV N antigen and neuronal markers in primary neurons at day 5 post-RSV infection. Primary neurons infected with RSV/A2 were fixed at 5 day p.i. RSV N protein was detected together with neuronal markers Nissl (A), MAP2 (B), PGP9.5 (C), and neurofilament (D). RSV N antigen staining was merged with each of the neuronal markers. RSV N antigen staining overlapped with each of the neuronal markers, indicating that RSV infects primary neurons (Magnification, ×32). No staining was detected in negative control neuronal cells (Fig. 1).