FIG. 3.
Detection of RSV N antigen in neuronal processes in lung tissue. Lung tissues from mice infected with 106 PFU RSV/A2, 6340WT, 6340ΔG, or A2/ΔCX3C virus were fixed and subjected to immunohistochemistry using monoclonal antibodies to RSV N protein or neurofilament. RSV N protein staining was merged with neurofilament staining. Overlapping staining was observed in only the lungs of mice infected with wild-type RSV (RSV/A2 and 6340WT), not in lungs of mice infected with mutant RSV (6340ΔG and A2/ΔCX3C) (Magnification, ×16).