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p28II associates with components of the transcription machinery. Immunoprecipitation using AU1 antibody was performed on 293T cells transfected with p28II-AU1, Tax-2, or both. Immune complexes were resolved by SDS-PAGE, followed by Western blot analysis using Tax-2-, Pol II-, or p28II-specific antibodies. Prior to immunoprecipitation, 10% of lysates were saved (Input) and blotted as above.
