Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HCV NS3 and NS5A proteins in Huh-7.5 cells containing replicons. Twenty micrograms of HCV RNA was electroporated into Huh-7.5 cells. At 4 h, cells were washed with PBS, scraped off the plates, lysed with 5% SDS, and analyzed with a 10% SDS gel. Antibodies against either (A) NS3 or (B) NS5A proteins were used for immunoblotting. Blots were stripped and reprobed with a β-actin antibody.
