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Long-term effects of the T450I and W501F mutations. (A) Amount of plus-strand HCV RNA detected per microgram of RNA isolated at different times from cells transfected with S2204I-Bsd, T450I, or W501F replicons. (B) Western blot analysis of NS3 and NS5A protein expression in Huh-7.5 cells 25 days after electroporation. In both panels Huh-7.5 cells without replicons that were grown over the same period of time (without blasticidin) are shown for comparison.
