Skip to main content

FIG. 2.

FIG. 2.

Western blot analyses of novel CCHF virus glycoproteins. SW-13 cells were infected with CCHF virus. Supernatants were harvested and concentrated. (A) Supernatants were run on a 3 to 8% NuPAGE gel, and antibody GP38/379-392 against aa 379 to 392 within the GP38-coding region of the CCHF virus glycoprotein precursor was used to probe the proteins. (B) Proteins were probed with a polyclonal monospecific antibody to the mucin region of CCHF virus strain IbAr10200. Lanes 1 and 2, supernatants were run on a 3 to 8% NuPAGE gel. Lane 3, supernatant was run on a 5% SDS-PAGE gel containing urea.