FIG. 5.

Effect of mutations on the generation of GP38. (A) Schematic of the mutations generated in the CCHF virus glycoprotein ORF. (B) SW-13 cells were transfected with the indicated constructs, and proteins were pulse-labeled with [³⁵S]cysteine for 30 min and chased for 5 h. The supernatant proteins were immunoprecipitated with MAb 6C11. WT, RRLL↓, and untransfected control were also treated with 50 μM dec-RVKR-CMK. Proteins were run on a 3 to 8% NuPAGE gel. (C) SW-13 cells were transfected with the indicated constructs, and proteins were pulse-labeled with [³⁵S]cysteine for 30 min and chased for 3 h. Proteins were immunoprecipitated with the antibody GP38/379-392 and then run on a 3 to 8% NuPAGE gel.