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Southern analysis of DNA isolated from the tPMP-1r transposon mutant ISP479R (lanes 1 and 3) and parental tPMP-1s, ISP479C (lanes 2 and 4), probed with a transposon-specific probe. Chromosomal DNA was digested with EcoRI (lanes 1 and 2) or PstI (lanes 3 and 4). A probe that hybridizes with the gene encoding the transposase of Tn551 detected a single 13-kb EcoRI fragment and a 10.2-kb PstI fragment in strain ISP479R that was not in parent strain ISP479C.
