FIG. 6.

Lysis genes of Bcep781. (A) Induction of XL1-Blue cells carrying pGemT-27 (open squares) or the vector without insert (black circles). Chloroform (chl) was added at the indicated time. (B) Predicted membrane topology and charge distribution of the Bcep781 gp30 holin. Predicted transmembrane domains are underlined, and charges are indicated above the appropriate amino acid residues. (C) Organization of the Bcep781 Rz and Rz1 embedded gene pair. The presumptive Shine-Dalgarno sequences for the two start codons are in bold. The cysteine residue predicted by LipoP to be the site of processing by the signal peptidase II is bold and underlined.