Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
RNase protection assays confirm temperature stress microarray analysis. Fifty micrograms of RNA from strain JRS4 that was grown to early log phase at 37°C (lane 1) and then shifted to 40°C for 5 min (lane 2), 10 min (lane 3), or 20 min (lane 4) was hybridized to [α-32P]UTP-labeled groEL (A), ctsR (B), clpC (C), purC (D), and ska (E) probes. Lane 5 contains a size marker. Arrows indicate the band of the size expected for the hybridization product with each probe.
