FIG. 3.

Transcription of SPy0229 is increased at pH 6.0 and at 40°C. RNase protection assays in which 50 μg of RNA from strain JRS4 was hybridized to the [α-³²P]UTP-labeled SPy0229 probe are depicted. (A) RNA from JRS4 was grown to early log phase at pH 7.5 (lane 1) and then shifted to pH 6.0 for 5 min (lane 2), 10 min (lane 3), or 20 min (lane 4). (B) RNA was harvested from JRS4 grown to early log phase at 37°C (lane 1) and then shifted to 40°C for 5 min (lane 2), 10 min (lane 3), or 20 min (lane 4). Lane 5 contains a size marker in both panels. Arrows indicate the band of the size expected for the hybridization product with the SPy0229 probe.