FIG. 5.

Acid-dependent increase in rscA transcript levels is largely CovR dependent. For the RNase protection assay, 50 μg of RNA from strain JRS4 or JRS948 was hybridized in duplicate to an [α-³²P]UTP-labeled rscA (SPy0229) probe. Each panel contains RNA from JRS4 (wild type [WT]) grown to early log phase at pH 7.5 (lanes labeled 1) and shifted to pH 6.0 for 5 min (lanes labeled 2) and RNA from JRS948 (ΔcovR) grown to early log phase at pH 7.5 (lanes labeled 3) and shifted to pH 6.0 for 5 min (lanes labeled 4). Lane 5 contains a size marker in both panels. Arrows indicate the band of the size expected for the hybridization product.